All Issue

2018 Vol.51, Issue 4 Preview Page
November 2018. pp. 491-498
Abstract

Biological control of plant disease using microorganisms such as Bacillus, Trichoderma and Streptomyces is alternative method which can reduce disease in ginseng cultivation. Antagonistic microorganisms can replace chemical agent, and reduce yield losses caused by several pathogens. The purpose of this study was to develop eco-friendly microbial agents and application technologies for the safe production of ginseng through control of ginseng aerial disease. For the selection of antagonistic microorganisms to control ginseng aerial disease, soil samples were collected from ginseng cultivation field, and 260 kinds of bacterial microbes were isolated. Among the 260 isolated microbes, KL87 strain suppressing the growth of ginseng pathogenic bacteria, Alternaria panax and Botrytis cinerea, was selected. As a result of 16S ribosomal RNA sequence analysis, KL87 strain was identified as Bacillus amyloliquefaciens. Furthermore, biochemical characteristics of KL87 strain showed high identity (99%) with Bacillus amyloliquefaciens. In the ginseng cultivation field test, Bacillus amyloliquefaciens KL87 reduced the occurrence of ginseng Alternaria blight by 75.9%. These results strongly suggested that Bacillus amyloliquefaciens KL87 could be used as microbial agents for replacement of chemical agent.

Suppressive effect of Bacillus amyloliquefaciens KL87 on Alternaria blight occurrence in ginseng cultivation field.

References
  1. Bae, Y.S., B.Y. Park, S.W. Kang, S.W. Cha, K.S. Hyun, B.Y. Yeun, T.J. Ahn, S.W. Lee, D.Y. Hyun, Y.C. Kim, K.C. Chung, S.K. Kim, and M.J. Han. 2005. Handbook of ginseng diseases and pests. National Institute of Crop Sciences Press. Suwon, Korea. pp.1-79.16181408PMC2683579
  2. Cho, J.S., S.G. Mok, and J.W. Won. 1998. Latest ginseng cultivation. Seonjin-muwhasa, Korea. p. 240.
  3. Jamal, Q., Y.S. Lee, H.D. Jeon, Y.S. Park, and K.Y. Kim. 2015. Isolation and biocontrol potential of Bacillus amyloliquefaciens Y1 against fungal plant pathogens. Korean J. Soil Sci. Fert. 48:485-491.10.7745/KJSSF.2015.48.5.485
  4. Kang, H.S., D.S. Park, Y.K. Hwang, and S.M. Kim. 2007. Survey on pesticide use by ginseng growers at Gangwon farmland in Korea. Korean J. Pestic. Sci. 11:131-218.
  5. Kim, B.Y., J.H. Ahn, H.Y. Weon, J.K. Song, S.I. Kim, and W.G. Kim. 2012. Isolation and characterization of Bacillus species possessing antifungal activity against ginseng root rot pathogens. Korean. J. Pestic. Sci. 16:357-363.10.7585/kjps.2012.16.4.357
  6. Kim, W.S. and J.S. Park. 2013. Selection and control effect of environmental friendly organic materials for controlling the ginseng Alternaria blight. Korean J. Med. Crop Sci. 21:388-393.10.7783/KJMCS.2013.21.5.388
  7. Kim, Y.S., M.S. Lee, J.H. Yeom, J.G. Song, I.K. Lee, W.H. Yeo, and B.S. Yun. 2012. Screening of antagonistic bacteria for biological control of ginseng root rot. Kor. J. Mycol. 40:44-48.10.4489/KJM.2012.40.1.044
  8. Korea patent.10-2002-0028954. Bacillus amyloliquefaciens LX 9 and method for culturing thereof.
  9. Lee, H.J., K.C. Park, S.H. Lee, K.H. Bang, H.W. Park, D.Y. Hyun, S.W. Kang, S.W. Cha, and I.M. Chung. 2012. Screening of antifungal Bacillus spp. against Alternaria blight pathogen (Alternaria panax) and anthracnose pathogen (Colletotrichum gloeosporioides) of ginseng. Korean J. Med. Crop Sci. 20:339-344.10.7783/KJMCS.2012.20.5.339
  10. Lee, S.K., J.S. Han, H.K. Kim, D.B. Yoon, and J.E. Choi. 2008. Control of Alternaria leaf blight of ginseng by microbial agent and fungicides. Res. Plant Dis. 14:102-106.10.5423/RPD.2008.14.2.102
  11. Lee, Y.D., K.A. Hussein, and J.H. Joo. 2017. Paenibacillus polymyxa and Burkholderia cepacia antagonize ginseng root rot pathogens. Korean J. Soil Sci. Fert. 50:598-605.
  12. Lim, J.S., D.W. Han, S.R. Lee, O.H. Hwang, J.H. Kwag, and S.B. Cho. 2015. A bacterial strain identified as Bacillus licheniformis using Vitek2 effectively reduced NH3 emission from swine manure. J. Anim. Environ. Sci. 21:83-92.10.11109/JAES.2015.21.3.83
  13. Lim, J.S., H.S. Mo, E.H. Lee, K.C. Park, and C.M. Chung. 2014. Suppressive effects of homemade environment-friendly materials on Alternaria blight and anthracnose of ginseng. Kor. J. Org. Agric. 22:705-718.10.11625/KJOA.2014.22.4.705
  14. Oh, S.H., Y.H. Yu, Y.H. Kim, G.H. Kim, and J.H. Lee. 1987. To study control of main disease to ginseng. Ginseng Research Report. Korea Ginseng Tobacco Research Institute. pp. 144-294.
  15. Park, K.H., H.W. Park, S.W. Lee, S.H. Lee, K.S. Myung, S.Y. Lee, J.K. Song, Y.T. Kim, K.S. Park, and Y.O. Kim. 2016. Isolation and characterization of Bacillus species having antifungal activity against pathogens of ginseng damping off. Korean J. Pestic. Sci. 20:380-387.10.7585/kjps.2016.20.4.380
  16. Rural Development Administration (RDA). 2009. Ginseng cultivation standard farming text book. Rural Development Administration. Suwon, Korea. pp. 94-109.
  17. Tamura, K., J. Dudley, and M. Nei. 2011. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version4.0. Mol. Biol. Evol. 24:1596-1599.10.1093/molbev/msm09217488738
Information
  • Publisher :Korean Society of Soil Science and Fertilizer
  • Publisher(Ko) :한국토양비료학회
  • Journal Title :Korean Journal of Soil Science and Fertilizer
  • Journal Title(Ko) :한국토양비료학회 학회지
  • Volume : 51
  • No :4
  • Pages :491-498
  • Received Date :2018. 09. 20
  • Accepted Date : 2018. 10. 28