The objective of this study was to evaluate nitrogen (N) source tracing using N stable isotope on corn (Zea mays L.) and leached water. Five nitrogen treatments were control (No nitrogen), 2 chemical N fertilizer (urea and slow released nitrogen (SRN), 1 cattle nitrogen manure (CM), and 1 oil cake from rapeseed (Brassica napus L.) after oil extraction. N rate was 200 kg N ha-1. The experiment was designed by a completely randomized block design with four replications. Lysimeter was filled with 4 kg of soil and corn was planted. Plant samples were collected at a growth stage of V6 and analyzed for total N and δ15N. Water samples were collected every week and analyzed for nitrate-N and δ15N. In this study, total N content at a growth stage of V6 corn plant ranged from 0.56% to 4.07%. N content was 0.56% for the control, 0.57% for the CM, 3.16% for the SRN, 3.19% for the oil cake, and 4.07% for the urea treatment. δ15N value at a growth stage of V6 corn plant ranged from 0.098‰ to -3.453‰. δ15N value in corn plant was -3.453‰ for the SRN treatment, -0.213‰ for the urea, 0.098‰ for the control, 2.935‰ for the oil cake, and 5.563‰ for the CM. Nitrate-N concentration in leached water was the lowest under control, followed by CM, SRM, oil cake, and nitrate-N concentration in leached was the highest under urea treatment. These results suggest different N mineralization from diverse N sources. Averagedδ15N isotope value in leached water changed from negative to positive. Averagedδ15N isotope under chemical N fertilizers such as urea and SRN was -16‰ and -15.0‰, respectively at the 5th week. Averagedδ15N isotope values for all N treatments changed during the experiment. These results indicate that δ15N isotope technique can be used for leached water samples to differentiate N sources. However, further research is needed to clarify the exact timing of sampling based on different N sources.
N treatment After N treatment 5th week 7th week δ15N ---------------------------------------------------- ‰ ---------------------------------------------------- Control NA+ NA Urea -16.6 ± 0.38‡ 0.1 ± 0.37 SRN -15.0 ± 0.91 -7.2 ± 1.13 Oil cake -13.2 ± 0.75 15.5 ± 1.9 CM -0.1 27.2
+NA is not available.
‡Standard Error (n=4), Number of observations of 15N value of CM at the 5th week and the 7th week is one each.
Leached soil water analysis for δ15N at the 5th week and the 7th week after N treatment in 2016.
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- Publisher :Korean Society of Soil Science and Fertilizer
- Publisher(Ko) :한국토양비료학회
- Journal Title :Korean Journal of Soil Science and Fertilizer
- Journal Title(Ko) :한국토양비료학회 학회지
- Volume : 51
- No :4
- Pages :420-426
- Received Date :2018. 08. 22
- Accepted Date : 2018. 11. 12